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One of the scientific approaches to correcting fibrotic myocardium is the development of regenerative medicine methods, which involve tissue therapy. The present study aimed to identify morphological and functional changes in the fibrous myocardium of rats after cryoinjury and the use of allogeneic biomaterial. The study included 80 Wistar rats divided into two groups, the control and the experimental group. All rats underwent a thoracotomy under general anesthesia with Xylazine and Zolazepam, and a section of the left ventricle was frozen with a cooled metal stylet. In the experimental group, a repeated thoracotomy was performed 45 days after cryodestruction. An allogeneic biomaterial suspension, dispersed from an acellular extracellular matrix with a particle size of 50 to 80 μm, was then injected intracardially into the area of cryogenic myocardial necrosis at a dose of 3 mg per animal. Forty-five days after the allogenic biomaterial injection, the tolerance of rats to physical activity was assessed using the modified Porsolt test. After the tolerance test, the weight of the heart, the thickness of the left ventricular wall, the thickness of the scar, and the diameter of the damaged area were measured. Three myocardium samples were taken from each animal and processed for examination using a light microscope. Histological examination of the myocardium samples revealed an avascular scar. In the experimental group, the thickness of the muscular part was significantly higher than in the control group. Meanwhile, heart weight didn’t differ much between the control and experimental group. The exercise tolerance of rats in the experimental group was higher than that of the control group and did not differ significantly from the intact values. The presented results supported the allogeneic biomaterial’s positive influence on the rats’ myocardium structure that had undergone cryogenic destruction.